Review



acsl4  (Boster Bio)


Bioz Verified Symbol Boster Bio is a verified supplier
Bioz Manufacturer Symbol Boster Bio manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Boster Bio acsl4
    Acsl4, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/Anti-COX2%2FCyclooxygenase+2%2FPTGS2+Antibody+Picoband/10__1016_slash_j__phymed__2026__158221-164-51-55
    Average 93 stars, based on 20 article reviews
    acsl4 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Over Expression:

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
    Article Snippet: bovine serum was purchased from Gemini. .. Anti-GPX4 antibody (BM5231), Anti-ACSL4 antibody (A04372-1), and Anti-PTGS2 antibody (A00084-2) were purchased from Boster. .. Anti-SLC7A11 antibody (BS66108) was purchased

    Labeling:

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
    Article Snippet: bovine serum was purchased from Gemini. .. Anti-GPX4 antibody (BM5231), Anti-ACSL4 antibody (A04372-1), and Anti-PTGS2 antibody (A00084-2) were purchased from Boster. .. Anti-SLC7A11 antibody (BS66108) was purchased

    Staining:

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
    Article Snippet: bovine serum was purchased from Gemini. .. Anti-GPX4 antibody (BM5231), Anti-ACSL4 antibody (A04372-1), and Anti-PTGS2 antibody (A00084-2) were purchased from Boster. .. Anti-SLC7A11 antibody (BS66108) was purchased

    Knockdown:

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
    Article Snippet: bovine serum was purchased from Gemini. .. Anti-GPX4 antibody (BM5231), Anti-ACSL4 antibody (A04372-1), and Anti-PTGS2 antibody (A00084-2) were purchased from Boster. .. Anti-SLC7A11 antibody (BS66108) was purchased

    Flow Cytometry:

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
    Article Snippet: bovine serum was purchased from Gemini. .. Anti-GPX4 antibody (BM5231), Anti-ACSL4 antibody (A04372-1), and Anti-PTGS2 antibody (A00084-2) were purchased from Boster. .. Anti-SLC7A11 antibody (BS66108) was purchased

    Expressing:

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import.
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import
    Article Snippet: The procedures for WB assays were described in the previous work.The procedures for WB assays were described in the previous work.. The anti-α-actinin antibody was from Sigma (A7732, Rahway, NJ, USA); the anti-β-Tubulin antibody was from Abclonal (A17073, Wuhan, China); the anti-ACSL4 antibody was from BOSTER (A04372-2); the anti-GPX4 antibody was from BOSTER (A02059-1); the anti-Fth1 antibody was from BOSTER (BM4487); the anti-NOX4 antibody was from BOSTER (BM4135, Wuhan, China); the anti-COX-1 antibody was from BOSTER (PB9002); the anti-Nrf2 antibody was from Proteintech (16396-1-AP, New York, NY, USA); and the anti-GSH antibody was from VIROGEN (101A, New York, NY, USA).. The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).The anti-Ubiquitin antibody was from Santacruz (sc-8017, Santa Cruz, CA, USA).

    Article Title: A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer
    Article Snippet: bovine serum was purchased from Gemini. .. Anti-GPX4 antibody (BM5231), Anti-ACSL4 antibody (A04372-1), and Anti-PTGS2 antibody (A00084-2) were purchased from Boster. .. Anti-SLC7A11 antibody (BS66108) was purchased



    Similar Products

    96
    Proteintech acsl4
    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, <t>ACSL4)</t> and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Acsl4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/ACSL4%2FFACL4+Antibody/pmc12963922-502-73-76
    Average 96 stars, based on 1 article reviews
    acsl4 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Huabio Inc anti acsl4
    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, <t>ACSL4)</t> and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Anti Acsl4, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/anti+dusp1/pmc13199173-87-7-8
    Average 86 stars, based on 1 article reviews
    anti acsl4 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Abmart Inc anti acsl4
    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, <t>ACSL4)</t> and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Anti Acsl4, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/acsl4+anti/pmc13069343-58-19-21
    Average 86 stars, based on 1 article reviews
    anti acsl4 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc acsl4
    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, <t>ACSL4)</t> and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Acsl4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/pmc12854050-170-69-71
    Average 86 stars, based on 1 article reviews
    acsl4 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Proteintech anti gpx4
    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, <t>ACSL4)</t> and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Anti Gpx4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/ACSL4%2FFACL4+Antibody/pmc12955691-78-109-110
    Average 96 stars, based on 1 article reviews
    anti gpx4 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Abmart Inc acsl4
    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, <t>ACSL4)</t> and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Acsl4, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/acsl4+anti/10__1016_slash_j__tdr__2026__100081-55-28-30
    Average 86 stars, based on 1 article reviews
    acsl4 - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    93
    Boster Bio acsl4
    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, <t>ACSL4)</t> and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Acsl4, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+acsl4+antibody/Anti-COX2%2FCyclooxygenase+2%2FPTGS2+Antibody+Picoband/10__1016_slash_j__phymed__2026__158221-164-51-55
    Average 93 stars, based on 1 article reviews
    acsl4 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration

    doi: 10.1016/j.bioactmat.2026.02.030

    Figure Lengend Snippet: D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: After blocked with 5% non-fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies against GAPDH (1:5000, 104941-AP, Proteintech), TSG101 (1:1000, DF8427, Affinity), CD9 (1:1000, AF5139, Affinity), CD63 (1:2000, 25682-1-AP, Proteintech), Calnexin (1:5000, 10427-2-AP, Proteintech), GM130 (1:20000, 11308-1-AP, Proteintech), CXCR3 (1:5000, 26756-1-AP, Proteintech), CXCL10 (1:2000, 10937-1-AP, Proteintech), MMP3 (1:2000, 17873-1-AP, Proteintech), ADAMTS5 (DF13268, Affinity), P16 (AF5484, Affinity), P21 (10355-1-AP, Proteintech), GPX4 (1:1000, 381958, Zen-bio), SLC7A11 (1:1000, 26864-1-AP, Proteintech), ACSL4 (1:5000, 22401-1-AP, Proteintech) and Tubulin (1:10000, T40103 , Abmart) overnight at 4 °C.

    Techniques: Western Blot, Staining, Control, Flow Cytometry